Chromotek gfp-trap® magnetic agarose
WebGFP-Trap® Magnetic Agarose Kit Product code: gtmak-20 Contact [email protected] ChromoTek GmbH Am Klopferspitz 19 82152 Planegg … WebThe ChromoTek GFP-Trap Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. It comprises a GFP Nanobody/ VHH coupled to magnetic agarose beads. …
Chromotek gfp-trap® magnetic agarose
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WebApr 11, 2024 · A 500 μl fraction of each cleared sample was mixed with 50 μl of pre-equilibrated GFP-Trap magnetic agarose (ChromoTek Cat No. gtma) or DYKDDDDK Fab-Trap Agarose (ChromoTek Cat No. ffa) and incubated over-night at 4°C rotating end-over-end. The beads were then washed twice with Wash buffer (10 mM Tris–HCl pH 7.5; 150 … WebJun 11, 2024 · ChromoTek GFP-Trap® Magnetic Agarose beads (other commercially available affinity purification systems can be used) and a magnetic separation rack. 2.3 Elution of Proteins 1. 0.5 M Ammonium hydroxide (pH > 10): Prepare solution the day of the experiment. 200 μL of solution is needed per sample. 2. Vacuum dryer apparatus.
WebGFP and Cfap91-GFP proteins were purified from the cytoplasm of GFP or Cfap91-GFP overexpressing cells using magnetic agarose beads-conjugated anti-GFP antibodies and after washings, incubated with a cytoplasmic extract obtained from cells overexpressing HA-Ccdc39 proteins (118 kDa). WebChromoTek pioneered the development and commercialization of alpaca Nanobodies. As a market and product leader for high quality and reliable Nanobody-based reagents, we assist scientists’...
WebJun 23, 2024 · ChromoTek. sku/catalog number : gtd-20. product description/product name : GFP-Trap Magnetic Particles M-270. Alias Product Names : GFP-Trap Magnetic Particles M-270. vendor tested application : Immunoprecipitation IP Co-Immunoprecipitation (CoIP) CoIP/MS RIP ChIP iCLIP Enzyme activity assays. WebJan 15, 2024 · ChromoTek's Nano-Traps are optimized for the immunoprecipitation (IP) of proteins and their interacting factors. Nano-Traps comprise of a Nanobody/ V H H conjugated to beads. For 3 Nano-Traps, e.g. GFP-Trap ® , RFP-Trap ®, and Spot-Trap ®, we offer different types of beads: Agarose beads Magnetic Agarose beads Magnetic …
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WebProteintech ChromoTek GFP-Trap™ Agarose GFP-Trap™ Agarose is an affinity resin for IP of GFP-fusion proteins. Brand: Proteintech GTA-20 Additional Details : Weight : 0.00240kg View more versions of this product Product Code. 17393343 378.00 € / 20 reactions Quantity Estimated Shipment: 28-03-2024 Add to basket Description … how does the sword in the stone work disneyWebApr 10, 2024 · Total cell extracts of yeast cells untreated or treated with CHX were incubated in ChromoTek GFP-Trap Agarose beads (Proteintech) for 2.5 h at 4 °C to pull-down AGP2-GFP tagged-associated proteins. After incubation, the beads coupled to proteins were washed with washing buffer (10 mM Tris/Cl pH 7.5, 0.5 mM EDTA, 150 … photogram photographyWebThe ChromoTek GFP-Trap™ Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. Brand: Proteintech GTMA-400 Additional Details : Weight : 0.00480kg View more versions of this product Product Code. 17353363 55828.28 NOK / 400 reactions Quantity Estimated Shipment: 12-05-2024 Add to basket Description Specifications how does the symbol of imdb differ from imdgWebgfp trap magnetic agarose beads - by Bioz Stars , 2024-03 86 / 100 stars Images 1) Product Images from "Myosin VIII and XI isoforms interact with Agrobacterium VirE2 … how does the supreme court reach a decisionWebBartlesville Urgent Care. 3. Urgent Care. “I'm wondering what the point of having an urgent care is if it's not open in the evening.” more. 3. Ascension St. John Clinic Urgent Care - … how does the surgery change charlieWebChromoTek GmbH. F2H®-Kit Basic: Kit comprises F2H®-BHK Cell Line, 1 vial 5 X 106 frozen cells (f2h-bhk); 100 µg Platform Reagent (f2h-p100); and 30 µg Control-DNA (f2h-c30) Platform Reagent: Transfection supplement enabling assembly of the nuclear PPI-platform to anchor GFP-tagged proteins; for 125 transfections (24-well format) or 500 ... how does the supreme court affect societyWebChromotek GFP-Trap is supposed to be superior beads. So if anyone can think as in how to promote the binding of the complex (GFP-SG1) of +HS samples to GFP-Trap, it would be very helpful. how does the supreme court get elected